Antioxidant evaluation and bio-guided isolation from methanol leaf extract of Acalypha godseffiana

Authors

  • S. D. Umoh Department of Chemistry, Department of Chemistry, Joseph Sarwuan Tarka University, Makurdi, Nigeria
  • A. K. Asekunowo Department of Chemistry, Faculty of Science, University of Lagos, Nigeria
  • I. S. Okoro Department of Chemistry, Department of Chemistry, Joseph Sarwuan Tarka University, Makurdi, Nigeria
  • N. X. Siwe Department of Chemistry, Faculty of Science, Rhodes University, South Africa
  • R. W. M. Kraus Department of Chemistry, Faculty of Science, Rhodes University, South Africa
  • O. O. Okoh Department of Chemistry, Faculty of Science and Agriculture, University of Fort Hare, Alice South Africa
  • A. O. T. Ashafa Department of Plant Science, Faculty of Natural and Agricultural Science, University of the Free State, South Africa
  • O. T. Asekun Department of Chemistry, Faculty of Science, University of Lagos, Nigeria
  • O. B. Familoni Department of Chemistry, Faculty of Science, University of Lagos, Nigeria

Keywords:

Acalypha godseffiana, spectroscopy, antioxidant, antifungal, bioactive compounds

Abstract

Acalypha godesffiana is a plant used in conventional medicine for fungal-related illnesses. The plant‘s extracts were investigated in this study, antioxidant, and antimicrobial studies were conducted. Different models were employed in the antioxidant assay; serial dilution was utilized to determine the minimum inhibitory concentration (MIC). The extract from A. godseffiana was purified and characterized by chromatographic and spectroscopic techniques, respectively. Three biologically active compounds, 3, 5-dihdroxylbenzoic acid (1), 3, 4, 5-trihydroxybenzoic acid (2), as well as Di-(2-ethylhexyl) phthalate (3), were identified for the first time in A. godseffiana. The extract and fractions exhibited varying scavenging capacities on different anti-oxidative models. The DPPH of MeOH (IC50= 0.51 mg mL^-1) was comparable with silymarine (SLY) IC50= 0.52 mg mL^-1 and better than gallic acid (GAL) IC50=1.95 mg mL^-1; the ABTS.+ of EtOAc column fraction (ACF, IC50=0.46 mg mL^-1) was comparable with standard SLY, IC50 = 0.47 mg mL^-1; and an OH radical of DCM, IC50= 0.10 mg mL^-1, was better than both standards (SLY, IC50= 6.30 mg mL^-1, GAL: IC50= 1.93 mg mL^-1). ACF showed superior antifungal activities (0.02 mg mL^-1) against Cryptococcus neoformans and Candida albicans, compared to ketoconazole (MIC of 0.250 mg mL^-1). Compounds (1-3) from A. godseffina reportedly displayed antioxidant and other activities. This study validated the antifungal potentials of A. godseffiana leaves and identified bioactive compounds. The extracts should be further investigated, and the compounds should be added to the existing library for further investigation of possible leads.

 

Dimensions

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Compounds identified from Acalypha godseffiana leaf extract.

Published

2024-06-19

How to Cite

Antioxidant evaluation and bio-guided isolation from methanol leaf extract of Acalypha godseffiana. (2024). Journal of the Nigerian Society of Physical Sciences, 6(3), 2038. https://doi.org/10.46481/jnsps.2024.2038

Issue

Section

Chemistry

How to Cite

Antioxidant evaluation and bio-guided isolation from methanol leaf extract of Acalypha godseffiana. (2024). Journal of the Nigerian Society of Physical Sciences, 6(3), 2038. https://doi.org/10.46481/jnsps.2024.2038